![]() If this does not solve your problem, please open a new issue to notify us, we are willing to help you solve the problem. If you have any questions about this application, please check: Macast/wiki/FAQ. ![]() Tutorials and examples are shown in: Macast/wiki/Custom-Renderer.įell free to submit a pull request to Macast-plugins. You can use a few lines of code to add support for other players like IINA and PotPlayer or even add additional features, like downloading media files while playing videos. You can modify the shortcut keys or configuration of the default mpv player by yourself, see: #how-to-set-personal-configurations-to-mpv.By loading the Macast-plugins, Macast can support third-party players like IINA and PotPlayer.įor more information, see: #how-to-use-third-party-player-plug-in.The ToF-SIMS images of Cl − and PO 3 − were acquired at m/ z 35 and 79, respectively.After opening this app, a small icon will appear in the menubar / taskbar / desktop panel, then you can push your media files from a local DLNA client to your computer. (N) and (O) Merged ToF-SIMS images of Cl − (red) and PO 3 − (green) and the CLSM image of EYFP-HMGB1 (blue) or DAPI (blue). (L) and (M) ToF-SIMS images of: (L) Cl − and (M) PO 3 − using 3000 BIB scans. ![]() (J) Merged image of the ToF-SIMS images of the total ions, Cl − and PO 3 −. (G)–(I) ToF-SIMS images of: (G) total ions (H) Cl − and (I) PO 3 − using 100 BIB scans. (E) Merged image of the CLSM images of EYFP-HMGB1 (green), DAPI (blue) and ToF-SIMS image of the total ions (red). (D) ToF-SIMS image of the total ions using 10 BIB scans. (B) and (C) CLSM images of (B) EYFP-HMGB1 and (C) the DAPI stained nucleus. ![]() (A), (F) and (K) Bright field images of a single cell framed in a red box: (A) before (F) after 10 cycles of GCIB sputtering on (A) and (K) after 100 BIB scans on (F). COSIMSi of lyophilized HeLa cells without treatment using cisplatin. This journal is © The Royal Society of Chemistry.įig. This finding suggests that Smad3 and its related signalling pathway are most likely involved in the intracellular response to cisplatin induced DNA damage. More significantly, for the first time, similar integrated imaging revealed that the cisplatin lesions at Smad-binding elements, for example GGC(GC)/(CG) and AGAC, disrupted the interactions of Smad3 with DNA, which was evidenced by the remarkable reduction in the expression of Smad-specific luciferase reporters subjected to cisplatin treatment. The superposition of the fluorescence and the mass spectrometry (MS) signals indicate the formation of HMGB1-Pt-DNA ternary complexes in the cells. We utilized confocal microscopy imaging to map EYFP-fused HMGB1 and fluorescent dye-stained DNA in single cells, followed by the visualization of cisplatin using high spatial resolution (200-350 nm) time of flight secondary ion mass spectrometry (ToF-SIMS) imaging of the same cells. Herein, we developed a dual-modal microscopy imaging strategy to investigate, in situ, the formation of ternary binding complexes of the transcription cofactor HMGB1 and transcription factor Smad3 with cisplatin crosslinked DNA in single cells. However, the way in which cisplatin-induced DNA lesions regulate interactions between transcription factors/cofactors and genomic DNA remains unclear. 6 College of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine Jinan 250355 People's Republic of China.Ĭisplatin is an extremely successful anticancer drug, and is commonly thought to target DNA.China 5 China Telecom Corporation Limited Beijing Research Institute Beijing 100035 People's Republic of China. 4 Beijing National Laboratory for Molecular Sciences, CAS Research/Education Center for Excellence in Molecular Sciences, Key Laboratory of Molecular Nanostructures and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences Beijing 100190 P.A refinement upgrade to the sophisticated SnowWolf vape systems, the new XFENG 230W TC Box Mod offers a phenomenal high-performance output chipset within a visually-striking chassis and robust OLED display. 3 University of Chinese Academy of Sciences Beijing 100049 People's Republic of China. or 4 interest-free payments of 11.99 with.1 Beijing National Laboratory for Molecular Sciences, CAS Research/Education Center for Excellence in Molecular Sciences, National Centre for Mass Spectrometry in Beijing, CAS Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences Beijing 100190 People's Republic of China 2 Key Laboratory of Hubei Province for Coal Conversion and New Carbon Materials, School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology Wuhan 430081 People's Republic of China.
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